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. 2017 Jul 15;130(14):2266–2276. doi: 10.1242/jcs.203000

Fig. 2.

Fig. 2.

When sister chromatid cohesion is lost, sister KTs exclude each other from sustained end-on MT attachment. (A) Diagrams summarizing the interaction of sister KTs with a single MT when cohesion is lost. When cohesion is lost and two sister KTs separate, a laterally attached sister KT detaches from the MT end after coming into contact with an end-on attached sister KT. (B) Representative example where sister KTs interact with a single MT after their cohesion is lost. This interaction was followed by detachment of one sister KT. Cells (T11941) with Scc1-AnchorAway PGAL-CEN3-tetO TetR-3×CFP GFP-TUB1 PMET3-CDC20 were treated as in Fig. 1D, except that rapamycin was added upon release from G1 arrest (to deplete Scc1). Images (GFP and CFP signals) were acquired every 10 s. The graph (right) shows the MT length and the position of CENs, as in Fig. 1C. Note that the spindle elongates after Scc1 depletion, although cells are arrested in metaphase (Tanaka et al., 2000). See Movie 3.