Fig. 3.

Some transformants have impaired polyphosphate signaling. (A) Cells were cultured with 150 µM polyphosphate for 4 h and proteasome activity levels were measured and normalized to no-polyphosphate (PolyP) controls. (B) Cells were cultured with 150 µM polyphosphate for 24 h and CsA levels were measured and normalized to no-polyphosphate controls. (C) Cells were cultured starting at 5×10⁵ cells/ml with or without 150 µM polyphosphate for 24 h, and the increase in cell number in the presence of polyphosphate was calculated as a percentage of the increase in cell number in the absence of polyphosphate. (D) Cells were cultured in HL5 and proteasome activity levels were measured and normalized to Ax2 controls. All values are mean±s.e.m., n≥3. *P<0.05, **P<0.01, ***P<0.001 (paired t-tests).