Figure 5.

Alteration of hsa-miR-1193 changed TGFBR2 mRNA and protein expression. (A) In HT-29 cells, TGFBR2 mRNA levels were repressed significantly by hsa-miR-1193 mimics. Upon inhibition of hsa-miR-1193, transcript levels of endogenous TGFBR2 increased. (B) Quantitative analysis showed that TGFBR2 protein expression in HT-29 cells transfected with hsa-miR-1193 mimics decreased compared with the mimics-NC. TGFBR2 protein increased in hsa-miR-1193 inhibitor transfected HT-29 cells compared with inhibitors-NC. (C) Representative pictures of TGFBR2 protein expression as detected by western blot. NC: negative control. **P < 0.01, ***P < 0.001.