Table 1.

Screening results of polymorphisms in miRNA target sites of sCRC-related genes SNPs (minor allele frequencies of 1–10%) were screened from the 3′-UTR regions of 31 sCRC-related genes. SNPs were selected based their occurrence within the miRNA seed region and their influence on miRNA targeting. miRNA recognition sites were perfect reverse complements to the seed region of miRNA sequences. The bold letters show polymorphisms in the recognition site. MFE change indicates the minimum free energy hybridization differences of the minor allele minus the major allele of the SNP. The symbol “+” indicates that a higher energy cost will be gained by miRNA binding with the minor allele, and “-” indicates a smaller cost gained. *SNP sites with MFE change higher than 3 kcal mol⁻¹ were considered for further research.

Candidate genes	SNPs in 3′UTR	Variation	Minor frequencies in Chinese	miRNA	miRNA recognition sites	MFE change
SMAD3	rs746978	G>T	T:0.089	hsa-miR-3200-5p	CTCAGAT	+5.9*
				hsa-miR-452-3p	CAGATGA	0
TGFBR2	rs6550008	A>G	G:0.012	hsa-miR-338-3p	ATGCTG	−0.3
	rs11466537	T>A	A:0.018	hsa-miR-1193	CCATCCC	+4.6*
MLH1	rs1558529	T>C	C:0.058	hsa-miR-3149	ATATGAA	−2.5
MLH3	rs12588766	G>A	A:0.012	hsa-miR-3920	UTGAG	+0.1
EPDR1	rs2722280	C>T	T:0.081	hsa-miR-483-5p	TCGTCTT	+1.4
INSR	rs1051690	A>G	G:0.023	hsa-miR-136-5p	AATGGAG	0
CD4	rs28990971	G>A	A:0.023	hsa- miR-1225-5p	GTGCCCA	0
TP53	rs4968187	C>T	T:0.047	hsa- miR-601	CTAGACC	+1.7