Figure 8. Exchange of the TMS by a CAAX-box decreases the packing density.
Cells were transfected with plasmids encoding for N-terminally myc-tagged syntaxin (myc-syntaxin), myc-syx-CAAX or myc-syx-ΔS-CAAX (see Figure 1 for details). Visualization on membrane sheets shows that also the CAAX-constructs form clusters (Figure 8—figure supplement 1). Constructs were analysed as described in Figure 6. (a) Microscopy images from fixed cells expressing the indicated constructs. Shown are magnified views from the images as overlays (blue, DAPI; green, myc-staining). (b) From the same transfections the western blot analysis is shown. Top, loading control (LC) using transferrin receptor staining; bottom, myc-signal. (c) Absolute epifluorescence signals (EF) related to the corresponding absolute western blot signals (WB). Values are given as means ± S.E.M. (n = 3), (t-test: p=0.021 for myc-syntaxin/myc-syx-CAAX; p=0.005 for myc-syntaxin/myc-syx-ΔS-CAAX; *p<0.05, **p<0.01).
Figure 8—figure supplement 1. Visualization of myc-syntaxin, myc-syx-CAAX and myc-syx-ΔS-CAAX clusters on membrane sheets imaged by STED microscopy.
