. Author manuscript; available in PMC: 2017 Jul 31.

Published in final edited form as: Exp Parasitol. 2002 Jan;100(1):12–16. doi: 10.1006/expr.2001.4676

TABLE II.

Monoclonal Antibody to CD36 Does Not Inhibit Plasmodium Sporozoite Invasion of HepG2 Cells

Expt. ^a	Treatment	Number of EEFs^b
1	Control	249 ± 4
	MAb 8A6	238 ± 13
2	Control	714 ± 53
	Mab 8A6	620 ± 22

Experiments 1 and 2 were performed with HepG2 cells (ATCC HB8065; Rockville, MD), plated in DMEM with 10% fetal calf serum in labtek chamber slides, and allowed to grow overnight. Plasmodium berghei sporozoites in medium with or without 100 μg/ml mAb 8A6 were added to HepG2 cells. After 2 h at 37°C, unattached sporozoites were removed, fresh medium was added, and 2 days later cells were fixed and exoerythrocytic forms (EEFs) were visualized with mAb 2E6 (directed against the liver stage of P. berghei) and goat anti-mouse Ig conjugated to horseradish peroxidase.

Number of EEFs per 20 fields. Each point was plated in triplicate and shown are the means with standard deviations.