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. 2017 Jul 10;7(8):1085–1091. doi: 10.1002/2211-5463.12248

Figure 1.

Figure 1

The gene for IL‐24 is the target of miR‐203a‐3p.1 in HCC cells. (A) IL‐24 expression levels in HCC cell lines (SMMC‐7721, HepG2, Huh7, Hep3B, 97L and 97H) and normal liver cell line (LO2) were examined by ELISA. (B) qRT‐PCR was used to analyze IL‐24 mRNA expression in CML cells. Huh7 and Hep3B cells were transfected with miR‐203a‐3p.1, miR‐425‐5p, miR‐200b‐3p, miR‐200c‐3p, miR‐429, miR‐140‐3p.2, miR‐205‐5p or miR‐29‐3p mimics and IL‐24 mRNA was assayed by qRT‐PCR. (C) The IL‐24 gene is predicted as a target of miR‐203a‐3p.1 using targetscan 7.0. (D) IL‐24 protein in HCC cells. Huh7 and Hep3B cells were transfected with miR‐203a‐3p.1 mimics and IL‐24 protein level was assayed by ELISA. (E) Luciferase activity of IL‐24 3′‐UTR and mutated IL‐24 3′‐UTR in HCC cells with miR‐203a‐3p.1 mimics transfection. Huh7 and Hep3B cells were transfected with wild‐type or mutant type of IL‐24 3′‐UTR combined with miR‐203a‐3p.1 mimics and luciferase activity was assayed with a dual luciferase assay system. **P < 0.01; *P < 0.05.