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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Apr 4;56(19):5349–5352. doi: 10.1002/anie.201701419

Figure 1.

Figure 1

SDS-PAGE of PBSL. Following a 30 min incubation, most of the eGFP-LPETG-SpyTag clarified lysates was captured with either a) SpyCatcher-SrtA or b) SrtA-SpyCatcher resin. Release of almost all captured protein is achieved with a 3h ligation reaction with 2mM GGG in reaction buffer (PBS + 50μM CaCl2, pH 7.4) at 37°C.