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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Apr 4;56(19):5349–5352. doi: 10.1002/anie.201701419

Figure 2.

Figure 2

PBSL capture efficiency. a) 10μM eGFP-LPETG-SpyTag clarified lysates were incubated with either SpyCatcher-SrtA or SrtA-SpyCatcher resin for varying times. Approximately 80% capture occurred within 10 min for both resins. b) Varying concentrations of eGFP-LPETG-SpyTag clarified lysates were incubated with either resins for 30 min. ∼80% maximal capture was retained for lysate concentrations as low as 0.5μM. Captured [eGFP-LPETG-SpyTag] were determined by incubating the resin with stripping buffer (PBS + 200mM imidazole, pH 7.4) and measuring eGFP absorbance at 488nm. Data are mean ± SD; n=3.