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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Apr 4;56(19):5349–5352. doi: 10.1002/anie.201701419

Figure 4.

Figure 4

SDS-PAGE comparing PBSL (Lanes 1-2) with traditional sortase ligation (Lanes 3-9) and STEPL (Lane 10). Following ligation with either PBSL in solution or sortase, a fluorescent band corresponding to the ScFv-LPETGGG-TAMRA product appears. PBSL results in significantly more fluorescently labeled product than either SrtA or STEPL. Traditional sortase ligation was carried out with SrtA:ScFv molar ratios ranging from 1:1 to 100:1. The ScFv-LPETGGG-TAMRA product for traditional sortase ligation runs slightly higher than that of PBSL due to a longer linker between the ScFv and the LPETG SrtA recognition motif.