(A–F) Changes in trabecular bone microarchitecture parameters throughout a reproductive cycle within the same trabecular VOI in reproductive rats (black triangles) and virgins (red squares). Letters indicate significant differences (p<0.05) among time points in the reproductive group (no significant changes over time were found for virgin rats). Intermediate time points (weeks 2, 4, 5, 7, 8, and 9) for virgin rats were not included in the analysis. (G) Representative 3D renderings illustrating changes in trabecular bone over the course of reproduction within a consistent trabecular VOI in a single rat.