Figure 6.

PIM2 inhibition by JP11646 has anti-myeloma activity in murine models. A) Pictures show mice with subcutaneous tumors of MM1.S at day 14 of treatment from groups vehicle (30% modified β-cyclodextrin), JP10 (JP11646 i.p. 10 mg/kg given consecutively 3 days /wk) or JP15 (JP11646 i.p. 15 mg/kg given 2 days/wk). B, Left) Plot of disease burden in mice followed over the entire experimental period of 48 days. For the sake of clarity, groups are staggered while plotting at each time point to avoid overlap. B, Right) Median survival (days) for the different groups with range (error bars) are shown. p-values were determined using the log rank (Mantel-Cox) test. C) Kaplan-Meier plots for survival was plotted using Graphpad Prism. D) Mice with 100–200 mm³ tumors were treated with a single dose of JP11646 at 15 mg/kg, and then tumors collected immediately or after 1,3 or 6 hrs, lysed and immunoassayed for PIM2, pBAD (S112), BAD, MCL1, pEBP1 (S65), total 4EBP1 or Tubulin. * p<0.05, ** p<0.01, ***p<0.001, NS-Not significant.