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. 2017 Jul 6;7(8):1154–1165. doi: 10.1002/2211-5463.12256

Figure 3.

Figure 3

Effects of DNA demethylation on miR‐205 expression and expression of DNMT family in MCF10A‐neo, MCF10A‐ErbB2, and MCF10A‐RafCAAX cells. (A) Real‐time quantitative RT‐PCR analysis of miR‐205 in MCF10A‐RafCAAX, MCF10A‐ErbB2, and MCF10A‐neo cells treated with the DNA methylation inhibitor. Cells were treated with 5‐aza‐2′‐deoxycytidine (5 μm) for 48 h. Data are normalized to vehicle control and represented as mean ± SEM of three independent experiments. *P < 0.01 by Student's t‐test compared with vehicle. (B) Western blot analysis of MCF10A‐RafCAAX, MCF10A‐ErbB2, and MCF10A‐neo cells, with antibodies for the indicated proteins. β‐Actin was used as a control for loading. (C) Western blot analysis of ZM‐336372‐treated MCF10A‐ErbB2 and MCF10A‐RafCAAX cells, with antibodies for the indicated proteins. β‐Actin was used as control for loading. Numbers below bands indicate the relative levels of DNMT1, 3a, or 3b normalized to β‐actin, calculated assuming level of untreated cells equal 1, as determined by densitometric analysis.