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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Nat Commun. 2014 Sep 12;5:4814. doi: 10.1038/ncomms5814

Figure 1. IP3R-Ca2+ store depletion promotes the DN to DP transition.

Figure 1

Expression of surface CD4 and CD8 (top row) in 1-week-old neonate (a) and 6-week-old adult (b) thymi of IP3R-TKO and control mice. Total thymic cellularity is shown above plots (mean±s.e.m.). CD8+CD4− thymocytes were subgated (red boxes) and examined for surface expression of CD24 and CD3 to observe ISP cells contained in the CD8+ CD4− populations shown in a and b. This was done in both neonatal (c) and adult (d) thymi of IP3R-TKO and control mice. Numbers in plots indicate percentage of cells. Absolute cellularity as depicted in a–d for neonatal (e) and (f) adult thymic subsets (mean±s.e.m.). All animals displaying signs of malignant disease were excluded from these analyses. Data are representative of a minimum of five (a–f) independent experiments. *P<0.05, measured with two-tailed Student’s t-test.