Table 1.

Mutations identified in exon 34 of the mouse Notch1 gene in IP3R-TKO thyme.

Mouse	Mutation (# of clones with mutation)	Variant			Protein effect	Nature of variant
		Insertion	Deletion	Mutation
A	1 (3/10)	7343-G		7344/45AC>GA	R2361EfsX130	Frameshift
B	2 (1/10)	7451-GC			S2397RfsX13	Frameshift
	3 (3/10)	7344-C		7346G>C	R2361PfsX130	Frameshift
C	4 (2/10)	7345-TT	7350-G	7346G>T	R2361LfsX130	Frameshift
	5 (5/10)	7345-TGTT			R2361LfsX131	Frameshift

RNA was isolated from individual tumour cells obtained from three different mice to generate cDNA. Primers to exon 34 of the mouse Notch1 gene were used to amplify the DNA sequence, which was then subcloned, and 10 clones from each mouse were sequenced. All identified variants are predicted to result in truncations of encoded Notch1 proteins (also refer to Supplementary Fig. 10).