The SV40 Late Gene Is Only Expressed in SuperVero Cells during the Production of Vector Particles and Not in Human Target Cells
Detection of GFP, SV40 LTag, and major capsid protein VP1 in cell lysates from SuperVero and HeLa cells transfected with pSVGFP DNA by western blot analysis using antibodies specific for GFP, LTag, and VP1. An anti-GAPDH antibody was used as a loading control.