Figure 2.

The Expression of miR-31 Correlates with the Amount of DNA Damage Incurred when Treated with Platinum-Based Chemotherapeutics

(A) Representative western blot time course for γH2A.X as a marker of DNA damage with cisplatin treatment (50 μM). Across all time points, it is evident that levels of γH2A.X decreased in miR-31-positive cells. (B) Representative western blot for γH2A.X with cisplatin treatment (50 μM). The confirmation of a reduction in DNA damage with miR-31 can be viewed in the second band (left to right), with an increase in DNA damage evident in the miR-31-suppressed P31 cell line. (C) Representative western blot for γH2A.X with carboplatin treatment (500 μM).