Figure 6.

Reintroduction of miR-31 Affects Lysosomal Drug Transport

(A) Expression levels of the drug influx transporter abcb9 were analyzed via qPCR. There is a significantly greater relative expression level (p = 0.0251) of ABCB9 in miR-31-transfected cells compared to the miR-VC-transfected equivalent (n = 4). RQ relates to relative fold change. (B) Representative western blot illustrating an increase in ABCB9 expression level with miR-31 re-expression, with no apparent change in the lysosomal marker LAMP1. (C) Densitometry analysis revealing significant (p = 0.0325) upregulation of ABCB9 (n = 3). (D) Mean signal intensities of NCI-H2452-transfected cells immunofluorescently stained with ABCB9 or LAMP1, with intensities captured in the Texas red channel for the images in (E). (E and F) Immunofluorescent images showing slight alteration in ABCB9 (E) or LAMP1 (F) staining (red); nuclei are stained with DAPI (blue). Images were captured on LSM 710 with a 63×/1.40 oil DIC M27 objective for ABCB9 images and a 10×/0.25 Ph1 objective for LAMP1 images. Data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.