Figure 7.

Gapmer AON Increased ECM Deposition of Collagen VI in Co-staining with Fibronectin in Fibroblast

(A) Double immunostaining of collagen 6 (green) and fibronectin (red) was performed in a patient’s unpermeabilized fibroblasts treated with scrambled AON (UCMD+AON-Scr) and AON-3 (UCMD+AON-3) and compared with staining in fibroblasts from a healthy control. Scale bar, 30 μm. (B) Semiquantification of the fluorescence signals from collagen 6, relative to the signals from fibronectin, was performed using ImageJ software. Three to four images were captured from each coverslip, and the experiment was performed in triplicate (*p < 0.05; **p < 0.01; ***p < 0.001; n = 9–12 images/group). Data are presented as mean ± SEM.