Fig. 5.

Hematological and immunological analyses. a–j Number and size of the different blood cell types were determined using a hematology analyzer (two-way ANOVA; young + AL: n = 16, young + EOD: n = 16, old + AL: n = 20, old + EOD: n = 22). a RBC count, b hemoglobin concentration, c HCT, d MCHC, e RDW, f platelet count, g platelet volume, h PDW, i large cell ratio of platelets (>12 fl), and j PCT. k–w Peripheral blood leukocytes were analyzed using a ten-color flow cytometer (two-way ANOVA; young + AL: n = 16, young + EOD: n = 16, old + AL: n = 20, old + EOD: n = 22). Only CD45-positive cells were included in the analysis. k T cells (CD3⁺CD5⁺), l Ly6C⁺ T cells, m B2 cells (CD5⁻CD19⁺B220⁺), n CD11b⁺ B cells (CD19⁺B220⁺), o Ly6C⁺ B cells (CD19⁺B220⁺), p natural killer (NK) cells (CD3⁻CD5⁻NKp46⁺ and/or NK1.1⁺), q CD11b⁺ NK cells, r CD11c⁺ NK cells, s NKT cells (CD3⁺CD5⁺NKp46⁺ and/or NK1.1⁺), t granulocytes (CD11b⁺Ly6G⁺), u Ly6C⁺ monocytes (CD11b⁺), v Ly6C⁻ monocytes (CD11b⁺), w non-specific cells, x plasma concentration of IgE immunoglobulins. AL ad libitum, EOD every-other-day feeding. Data are shown as mean ± SEM. Full data sets of hematological and immunological analyses are presented in Supplementary Tables 14 and 15