Fig. 6.

Clinical chemistry measures in plasma of fed and fasted mice. Plasma concentrations of a sodium, b potassium, c urea, d glucose, and e albumin, f unsaturated iron-binding capacity, as well as activities of g alkaline phosphatase (AP) and h lactate dehydrogenase (LDH) were determined in fed mice (two-way ANOVA; young + AL: n = 16, young + EOD: n = 16, old + AL: n = 20, old + EOD: n = 22). Plasma concentrations of i fasting NEFA and j fasting insulin were measured in animals after a 6 h fasting period (two-way ANOVA; young + AL: n = 16, young + EOD: n = 16, old + AL: n = 23, old + EOD: n = 23). k Blood glucose concentration during an IpGTT (three-way ANOVA with the between-subjects factors age (young vs. old), DR (AL vs. EOD), and the within-subjects factor time points (Greenhouse-Geisser correction); young + AL: n = 16, young + EOD: n = 16, old + AL: n = 23, old + EOD: n = 23). AL ad libitum, EOD every-other-day feeding. Data are presented as mean ± SEM. Full clinical chemistry data sets are presented in Supplementary Tables 16 and 17