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. 2017 Jul 31;7:6874. doi: 10.1038/s41598-017-07269-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of silencing Hs-Tyr on H. schachtii parasitism. The graphs show the results of bio-assays with J2 soaked in Hs-Tyr -specific dsRNA compared with J2 soaked in GFP dsRNA as a negative control. The following parameters were analysed: (a) number of males and females (b) total number of nematode infections (c) female size (d) size of syncytia. Data are based on three independent experiments. Each bar represents the mean ± standard error of n > 35. Asterisk indicate significant differences based on Student’s t-test (P < 0.05).