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. 2017 Jul 31;7:6926. doi: 10.1038/s41598-017-07512-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TPPU administration regulated Calcineurin/NFATc3 signaling in adventitial remodeling mice. (A) Representative immunoblots and quantitation of CnA protein expression in aortas. (B) Measurement of CaN phosphatase activity in aortas. (C) Representative immunoblots and quantitation of p-NFATc3/NFATc3 expression in aortas. (D) Representative immunoblots and quantitation of NFATc3 nuclear translocation in aortas. Data were expressed as Mean ± SEM, n = 8 mice for each group. *p < 0.05 vs. Vehicle, ^#p < 0.05 vs. Ang II, **p < 0.05 vs. Vehicle (Cytoplasm), ^##p < 0.05 vs. Ang II (Cytoplasm), ^&&p < 0.05 vs. Ang II + TPPU (Cytoplasm), ^$$p < 0.05 vs. Vehicle (Nucleus), ⁺⁺p < 0.05 vs. Ang II (Nucleus).