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. 2017 Jul 31;7:6926. doi: 10.1038/s41598-017-07512-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of TPPU pretreatment on Ang II-induced AFs activation. (A) Immunofluorescent staining of Vimentin and α-SMA in AFs (Red: Cy3, Green: FITC, blue: DAPI). (B) Representative immunoblots and quantitation of α-SMA and Collagen I protein expression in Ang II-stimulated AFs pretreated with or without TPPU. (C) Measurement of cell proliferation by CCK-8 assay in Ang II-stimulated AFs pretreated with or without TPPU. (D) Measurement of cell migration by transwell assay in Ang II-stimulated AFs pretreated with or without TPPU. Data were expressed as mean ± SEM, n = 4 for each group, *p < 0.05 vs. Control, ^#p < 0.05 vs. Ang II.