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. 2017 Jul 31;7:6870. doi: 10.1038/s41598-017-07167-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Slco2a1, Ptger2, Ptger4, Dspp, nestin, and Vegfa mRNA expression in rat first molars after pulpotomy followed by MTA capping. Real-time PCR used to quantify Slco2a1 (a), Ptger2 (b), Ptger4 (c), Dspp (d), nestin (e), and Vegfa (f) mRNA levels in normal first molar tissue and at each examined time point after pulpotomy. Data show the mRNA expression levels of Slco2a1, Ptger2, Ptger4, Dspp, nestin, and Vegfa normalized to β-actin mRNA levels. (a) The increased expression of Slco2a1 mRNA is maintained to 7 days. (b) Expression of Ptger2 mRNA falls to normal at 3 days and thereafter. Ptger4 (c), Dspp (d), and nestin (e) mRNA expression levels gradually increase in a time-dependent manner after operation. (f) The expression of Vegfa mRNA steadily decreases in a time-dependent manner, although significantly higher expression than the normal level is maintained. Bars represent mean values ± standard error of the mean, compared with the normal control (n = 6; *P < 0.05 and **P < 0.01).