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. 2017 Jul 31;7:6885. doi: 10.1038/s41598-017-06969-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hesperidin treatment preserved RGC marker-positive cells after NMDA injury. Representative photographs at lower (a–c) and higher (d–l) magnification with nuclear staining (DAPI) and immunostaining with anti-RBPMS antibody, taken 24 h after intravitreal injection with PBS (a,d,g,j), 30 nmol NMDA (b,e,h,k), or NMDA with hesperidin (c,f,i,l). RBPMS-positive cells in the GCL are shown by the arrows. (m) Histogram showing the average number of RBPMS-positive cells in the retinas. Data represent mean ± SD, (vehicle, n = 6, NMDA, n = 8, NMDA with hesperidin, n = 7) ***p < 0.001. A-C scale bar: 500 µm, D-L scale bar: 50 µm. GCL; ganglion cell layer, INL; inner nuclear layer, ONL; outer nuclear layer.