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. 2017 Jul 31;7:6884. doi: 10.1038/s41598-017-05097-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Variations of BC200 RNA levels in cancer cells. Total cellular RNA was purified from cervical HeLa cells, breast cancer cell lines (MCF7, SK-BR-3, MDA-MB-231, MDA-MB-435, Hs578T, and T47D), the normal breast cell line, MCF10A, and the normal keratinocyte cell line, HaCaT. (A) The endogenous expression levels of BC200 RNA and H1 RNA were assessed by Northern blotting. The spliced images from the same Northern membranes were shown with the insertion of dividing lines between spliced lanes. (B) The Northern results were normalized with respect to the expression of the 5S rRNA. (C) Quantitative analysis of BC200 RNA abundance. Total cellular RNAs were subjected to qRT-PCR using 18S rRNA as a control RNA. BC200 (Endo), endogenous BC200 RNA.