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. 2017 Jul 31;7:6884. doi: 10.1038/s41598-017-05097-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Half-lives of BC200 RNA deletion or mutant derivatives. (A) Schematic diagrams of BC200 RNA (nts 1–200). Deleted sequences were shown in orange for RNAΔ98-117 and purple for RNAΔA. Mutated sequences were also shown in blue for RNA(A-Box)m and red for RNA(B-Box)m. Alu domain (nts 1–122), A-rich region (nts 123–158) and C-rich region (nts 159–200) were indicated. Total cellular RNA was isolated from HeLa cells transfected with −100 nt upstream constructs expressing BC200 RNA mutants or deletion derivatives, and the half-lives of the BC200 RNA mutant (B) or deletion (C) derivatives were analyzed as described in the legend to Fig. 6. The cells were transfected with 1 µg of plasmids expressing BC200 RNA or its derivatives. The remaining BC200 RNA levels (ln[BC200]/[BC200]t = 0) are also plotted versus time in hours. BC200 (Ecto) and BC200 (Exo) stand for ectopic and exogenous BC200 RNA, respectively.