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. 2017 Jul 31;7:6884. doi: 10.1038/s41598-017-05097-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptional activities from the transfected BC200 RNA gene. (A) Various cell lines were transfected with constructs containing different 5′ upstream sequences intended to drive the expression of RNAΔA. Total cellular RNAs were purified and subjected to Northern blotting. The same molar ratio of RNAΔA-expressing construct to M1 RNA-expressing construct was used for each transfection. The amount of plasmid DNAs expressing RNAΔA are indicated above the lanes. Each hybridized membrane included the same total RNA preparation from HeLa cells (HeLa*), and the Northern signal from this sample was used to standardize the data across different hybridization membranes. Serial dilutions (1/1.7, 1/2, 1/3.3, 1/7 or 1/14) of total cellular RNA were used for semi-standard curves. (B) The relative transcriptional activities of RNAΔA were calculated by dividing its Northern signals by that of the M1 RNA, after both signals were normalized with respect to the 5S rRNA.