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. 2017 Jul 31;7:6884. doi: 10.1038/s41598-017-05097-3

Table 1.

Cell-specific features in BC200 RNA biosynthesis.

Cell line Cellular levela Half-life (h)b Relative promoter activity of transfected pT1010BC∆A DNAc
Northern blot qRT-PCR Endogenous Exogenous
SK-BR-3 185 ± 15 39 ± 6 NDd ND 9.7 ± 0.4
MCF10A 100 ± 26 100 ± 19 ND 7.9 100 ± 30
MDA-MB-231 178 ± 40 108 ± 3 ND 4.3 71 ± 13
HaCaT 206 ± 69 321 ± 118 26.6 6.9 105 ± 30
T47D 305 ± 82 946 ± 63 13.6 7.1 354 ± 121
HeLa 302 ± 101 862 ± 31 >200 12.5 178 ± 53
Hs578T 371 ± 142 870 ± 253 40.1 5.4 85 ± 13
MDA-MB-435 572 ± 152 1400 ± 288 4.2 4.1 196 ± 26
MCF7 658 ± 101 1413 ± 44 5.3 5.1 166 ± 13

aCellular levels of BC200 RNA were calculated from the data of Fig. 5B (for Northern blot) and Fig. 5C (for qRT-PCR).

bHalf-lives of BC200 RNA were determined by linear regression analysis from the data of Fig. 6A (for endogenous) and Fig. 6B (for exogenous).

cRelative promoter activities were from the data of Fig. 8.

dND, not determined.