Table 1.
Cell line | Cellular levela | Half-life (h)b | Relative promoter activity of transfected pT1010BC∆A DNAc | ||
---|---|---|---|---|---|
Northern blot | qRT-PCR | Endogenous | Exogenous | ||
SK-BR-3 | 185 ± 15 | 39 ± 6 | NDd | ND | 9.7 ± 0.4 |
MCF10A | 100 ± 26 | 100 ± 19 | ND | 7.9 | 100 ± 30 |
MDA-MB-231 | 178 ± 40 | 108 ± 3 | ND | 4.3 | 71 ± 13 |
HaCaT | 206 ± 69 | 321 ± 118 | 26.6 | 6.9 | 105 ± 30 |
T47D | 305 ± 82 | 946 ± 63 | 13.6 | 7.1 | 354 ± 121 |
HeLa | 302 ± 101 | 862 ± 31 | >200 | 12.5 | 178 ± 53 |
Hs578T | 371 ± 142 | 870 ± 253 | 40.1 | 5.4 | 85 ± 13 |
MDA-MB-435 | 572 ± 152 | 1400 ± 288 | 4.2 | 4.1 | 196 ± 26 |
MCF7 | 658 ± 101 | 1413 ± 44 | 5.3 | 5.1 | 166 ± 13 |
aCellular levels of BC200 RNA were calculated from the data of Fig. 5B (for Northern blot) and Fig. 5C (for qRT-PCR).
bHalf-lives of BC200 RNA were determined by linear regression analysis from the data of Fig. 6A (for endogenous) and Fig. 6B (for exogenous).
cRelative promoter activities were from the data of Fig. 8.
dND, not determined.