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. 2017 Jul 31;7:6966. doi: 10.1038/s41598-017-07294-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TUNEL positive cells in the 4 experimental groups 6 days post surgery. Representative images of retinas from sham-operated (A), sham-operated treated with hypothermia (B), IONC-operated (C), and IONC-operated rats treated with hypothermia (D). TUNEL positive cells were found mainly in the ganglion cell (GC) layer (arrowheads) and in the inner nuclear layer (INL, arrows). ONL: outer nuclear layer. Bar = 50 µm. Quantification of the results at 3 time points after surgery are shown as histograms (E). Bars represent the mean ± SD of all samples (n = 5 per group). Asterisks represent statistically significant differences. *p < 0.05; ***p < 0.001; ****p < 0.0001.