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. 2017 Jul 31;7:6959. doi: 10.1038/s41598-017-07244-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-labeling MPM imaging of human normal colorectal mucosa. (a) Overview of the imaging approach. Colorectal tissue was placed on a rubber plate with the mucosal surface facing upwards using small pins and was overlaid with a coverslip (arrow) to form a drop of water (arrow head). (b) MPM imaging of human normal colorectal mucosa. (c) HE staining of normal colorectal mucosa, using the same sample as in (b). (d–f) Images obtained using a 417/60 nm filter (d), 480/40 nm filter (e), and 629/56 nm filter (f) of the samples used in (b). Fibrous structures (arrow), epithelial cells (arrow head), and immune cells (asterisk) showed different color patterns. Bar: 50 µm.