Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 31;7:6959. doi: 10.1038/s41598-017-07244-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Spectral analysis and immunohistochemical staining of normal colorectal mucosa. (a–d) Spectral analyses under excitation at 730 nm (a), 820 nm (b) and 900 nm (c) of the ROIs in the MPM image from normal colorectal mucosa (d). The green line is the spectrum of the epithelial cell region (green square ROI with a side length of 15 µm in (d)); red color indicates the immune cell region (red ROI); and the yellow color indicates the fibrous structure region (yellow ROI). (e) Immunohistochemical staining of normal colorectal mucosa using an anti-FAD antibody. Brown staining (FAD positivity) is distributed primarily in immune cells in the lamina propria (arrow). (f) Immunohistochemical staining using an anti-laminin antibody. The distribution of laminin-positive staining in the basement membrane (arrow head) is consistent with the fibrous structures observed by MPM imaging derived from SHG signals. Bar: 50 µm.