Table 1.

Real time PCR primers used to quantify mRNA expression from human genes.

Gene
RUNX-2	Forward	5′-GGT CAG ATG CAG GCG GCC-3′
	Reverse	5′-TAC GTG TGG TAG CGC GTC-3′
SOX-9	Forward	5′-AGA CAG CCC CCT ATC GAC TT-3′
	Reverse	5′-CGG CAG GTA CTG GTC AAA CT-3′
Aggrecan (ACAN)	Forward	5′-TAC ACT GGC GAG CAC TGT AAC-3′
	Reverse	5′-CAG TGG CCC TGG TAC TTG TT-3′
Collagen type II (COL2A1)	Forward	5′-GTG AAC CTG GTG TCT CTG GTC-3′
	Reverse	5′-TTT CCA GGT TTT CCA GCT TC-3′
Collagen type X (COL10A1)	Forward	5′-CAC CTT CTG CAC TGC TCA TC-3′
	Reverse	5′-GGC AGC ATA TTC TCA GAT GGA-3′
GAPDH	Forward	5′-ACC CAG AAG ACT GTG GAT GG-3′
	Reverse	5′-TTC TAG ACG GCA GGT CAG GT-3′