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. 2017 Jul 31;7:6889. doi: 10.1038/s41598-017-05930-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LPS triggers lipid peroxidation and ethylene formation in leukocytes. (a) Lipid peroxidation in moDCs as measured by blue-shift of Bodipy581/591-C11 fluorescence. MoDCs were treated with 1 µg/ml LPS or 100 µM Fe(II)SO₄ (n = 4). (b) Lipid peroxidation in moDCs measured by EIA assay for 8-isoprostane. Cells were treated with either LPS or 100 µM Fe(II)SO₄ in serum-free RPMI-1640 (n = 3). (c) Total ethylene released during 1 hour stimulation of leukocytes with either 1 µg/ml LPS or 100 µM Fe(II)SO₄, as detected by laser-based photoacoustic spectroscopy (n = 11). Data points show mean with SEM (panels a and b) or geometric mean with 95% CI (panel c), asterisks indicate significance compared to untreated samples.