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. 2017 Apr 28;234(16):2399–2407. doi: 10.1007/s00213-017-4627-4

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Surgical preparation and immunohistochemical verification of anatomical targets. a Sagittal view of the rat brain indicating sites of virus injection (AAV2-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine) in the Pf and CNO in the DMS. b Left panel shows diagram of coronal view of the intralaminar thalamic nuclei with green eclipses indicating individual virus diffusion areas. Right panel shows confocal image of a thalamic slice indicating midline and intralaminar thalamic nuclei with GFP indicating expression of the DREADD (arrow). Inset shows Pf in higher magnification. c Left panel shows a coronal view of striatum indicating individual cannula tracks. Right panel shows a confocal image of striatum with injection tracks indicated. Inset shows DMS in higher magnification to show presence of GFP indicating DREADD expression in Pf-DMS axonal processes (marked with arrows)