Figure 8.

In vitro repair of a single nucleotide BER intermediate. Top, schematic representation of the formation of the BER intermediate used as substrate. The [³²P]3′-labeled 2′-deoxyuridine-containing substrate (lane a) was treated with 27 nM E. coli UDG (lane c), leaving a 5′-dRP terminus. The resulting BER intermediate (1 nM) was incubated with 3 nM of the indicated PgVPolX in the absence (−) or presence (+) of 10 µM dCTP for 10 min at 30 °C, as described in Materials and Methods. Position of products is indicated. Asterisk indicates an electrophoretic artifact that appears specifically with the 34mer product with an AP site at position 15.