Figure 1.

Resveratrol inhibits proliferation without modifying cell viability. (A) Caco2 cells were seeded into 96 well plates at 7 500 cells/cm² in DMEM containing 25 mM glucose and supplemented with 5% charcoal-treated serum. Twenty four hours after the plating, cells were treated with 10 µM resveratrol for 24, 48 and 72 hr. Cell proliferation was measured with BrdU according to the manufacturer’s recommendation. (B) Caco2 cells were treated with 10 µM resveratrol (RES) for 48 hr and stained with propidium iodide (PI). Quantitative analyses of the percentage of the cells in different phases of the cell cycle were performed by FACS analysis. (C) Caco2 cells were treated with 10 µM resveratrol for 48 hr and quantitative analysis of the percentage of the cells positive for annexin V and PI staining were performed by FACS analysis. (D) Caco2 cells were treated with 10 µM resveratrol for 48 hr and cell viability was evaluated by MTS assay. The means ± SEM of at least three independent experiments are shown (*p < 0.05, **p < 0.01 vs control, ***p < 0.001 vs control).