Figure 10.

AMPK signaling is involved in resveratrol-induced metabolic shift in colon cancer cells. Subconfluent colon cancer cells were treated with 10 µM of compound C (CC), an AMPK inhibitor (A) or 25 µM of STO-609 (STO), a CAMKK inhibitor (C,D), 1 hour before and during a 48 hour-treatment with 10 µM resveratrol (RES). Cells were isolated and 10⁶ cells were incubated in Krebs-Ringer phosphate buffer supplemented with 5 mM [U¹⁴C]-glucose (isotopic dilution 1/1000). ¹⁴CO₂ was recovered as described in the Material and Methods section. (B) Total cell lysates were collected from Caco2 cells treated with 10 µM resveratrol for 30 minutes and 1 hour, using RIPA buffer supplemented with protease and phosphatase inhibitors. Lysates were analyzed by Western blot using antibodies against P-AMPK(T172) and AMPK. Results are the means ± SEM of at least 3 independent experiments each performed in triplicate (*p < 0.05, **p < 0.01, ***p < 0.001 vs control).