Figure 5.

Resveratrol modulates the lipidomic profile of colon cancer cells. Caco2 cells from 12 different passages were treated with 10 µM resveratrol (RES) for 48 hr. After lipid extraction according to Bligh and Dyer⁵⁷, lipid extracts were analysis as described in Materials and Methods. (A) PCA score plots of resveratrol treated- (Δ, n = 12) and control cells (■, n = 12) were built from first and second component of PCA models generate from data acquired in UPLC-MS in ESI + (left) and ESI- (right) ion modes. Distribution of lipid species with (B) saturated (SFA), (C) monounsaturated (MUFA) and (D) polyunsaturated (PUFA) fatty acid side chains, in lipids showing an increased (dark grey bars) or a decreased (light grey bars) relative abundance following an exposure to resveratrol (10 µM, 48 hr).