Figure 7.

Resveratrol modulates PDH actvity in cancer cells. Caco2 cells were cultured at subconfluence in 5% charcoal-treated serum in DMEM 24 hr before and during the treatment with 10 µM resveratrol (RES) for 48 hr. (A) Total cell lysates were collected using RIPA buffer supplemented with protease and phosphatase inhibitors and analyzed by Western blot using antibodies against P-PDHE1α(S232), P-PDHE1α(S293) and P-PDHE1α(S300), and PDHE1α and actin for normalization. Data are the means ± SEM of at least 3 independent experiments (*p < 0.05, **p < 0.01). (B) Caco2 cells were cultured at subconfluence in 5% charcoal-treated serum in DMEM 24 hr before and during the treatment with 10 µM resveratrol for 48 hr. PDH activity was assayed by the release of ¹⁴CO₂ from [¹⁴C₁]-pyruvate according to Geoffroy et al.⁵⁸, as described in the Material and Methods section. Data are the means ± SEM of 6 independent experiments (***p < 0.001).