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. 2017 Jul 31;7:6945. doi: 10.1038/s41598-017-07006-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolic effects of resveratrol on glucose metabolism are related to a modulation of calcium flux in colon cancer cells. Subconfluent colon cancer cells were treated with 5 µM EGTA-AM (A, n = 3), 5 µM BAPTA-AM (B, n = 3) or 5 µM Ruthenium Red (C, n = 3; D, n = 4), 1 hour before and during a 48 hour-treatment with 10 µM resveratrol (RES). Cells were isolated and 10⁶ cells were incubated in Krebs-Ringer phosphate buffer supplemented with 5 mM [U¹⁴C]-glucose (isotopic dilution 1/1000). ¹⁴CO₂ was recovered as described in the Material and Methods section.