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. 2017 Jul 31;7:6945. doi: 10.1038/s41598-017-07006-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calcium modulates resveratrol-induced increase of PDH activity. (A) Caco2 cells were treated 5 µM RR and then with 10 µM resveratrol (RES) for 48 hr. PDH activity was measured as in the Material and Methods section (n = 4 to 8). Total cell lysates were collected from cells using RIPA buffer supplemented with protease and phosphatase inhibitors. Lysates were analyzed by Western blot using antibodies against (B) P-PDHE1α (S232) (n = 3) and (C) P-PDHE1α (S293) (n = 6). (*p < 0.05, **p < 0.01, ***p < 0.001 vs control).