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. 2017 Jul 31;7:6951. doi: 10.1038/s41598-017-07534-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in IOP observed in the AOH model and sham-operated rats. IOPs were evaluated in rats once every 20 minutes during irrigation and once every 2 hours after the irrigation using a hand-held, non-contact tonometer. The normal IOP of the rats was 10.2 ± 0.4 mmHg. At the beginning of irrigation, the IOP rapidly increased to 82.6 ± 2.3 mmHg (A). The IOP remained stable at 10.2 ± 1.0 mmHg in rats that underwent the sham procedure (B). After the infusion needle was removed, the IOP decreased to a level below the normal level and recovered to its baseline level after approximately 6 hours (A and B).