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. 2017 Jul 31;7:6951. doi: 10.1038/s41598-017-07534-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Peripheral CEC density quantification and histology. AOH decreased the peripheral corneal ECD (P < 0.05, Dunnett’s test) (A). Alizarin red staining revealed the CEC morphology (B). Normal CECs had a regular hexagonal morphology. Rapidly after irrigation, the morphology of CECs became irregular and multiform. Although the peripheral ECD increased to a normal level, the morphology was still not uniform after two days, whereas the morphology and size of the CECs had completely reversed to the normal condition after 7 days.