Fig. 1.

UCP2 over-expression in ß-cells of islets exposed to glucotoxic conditions. WT and RIP-UCP2 islets were collected for analysis 7 days after culture at either 11 mM glucose (11 mM) or 30 mM glucotoxic condition (30 mM). (A) UCP2 protein levels, normalized to ß-actin, were assessed by immunoblotting. Bar-graph shows means ± SE of 3 independent experiments, p < 0.05 versus WT. (B) binocular observation of islets at the end of the culture period.