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. 2017 Jul 21;13:541–549. doi: 10.1016/j.redox.2017.07.012

Fig. 2.

Fig. 2

Effects of UCP2 over-expression on mitochondrial membrane potential in islets exposed to glucotoxic conditions. At the end of the 7-day culture period at either standard 11 mM glucose (11) or glucotoxic condition (30), WT and RIP-UCP2 islets were assayed for mitochondrial membrane potential. (A) WT and RIP-UCP2 islets from the standard 11 culture conditions were monitored first at low 2.8 mM Glc followed by acute stimulation with 22.8 mM Glc (Glucose). (B) WT and RIP-UCP2 islets from either standard 11 or glucotoxic 30 culture condition were monitored at low 2.8 mM Glc before complete depolarization of the mitochondrial membrane induced by the addition of 1 µM FCCP. (C, D) WT (C) and RIP-UCP2 (D) islets from either standard 11 or glucotoxic 30 culture condition were monitored first at low 2.8 mM Glc followed by acute stimulation with 22.8 mM Glc (Glucose). Values are means from 3 independent experiments (n = 11–14 islets); *p < 0.05 wt 30 versus WT 11, #p < 0.05 RIP-UCP2 30 versus RIP-UCP2 11.