Fig. 7.

Effects of UCP2 over-expression on superoxide generation in islets exposed to glucotoxic conditions. At the end of the 7-day culture period at either standard glucose (11 mM Glc) or glucotoxic condition (30 mM Glc), superoxide generation was measured in WT and RIP-UCP2 islets either immediately at the end of the 7-day culture (A) or following further glucose challenge (B). (A) superoxide levels from control (11 mM Glc) and glucotoxic (30 mM Glc) conditions in both WT and RIP-UCP2 islets. *p < 0.05 versus WT from the 11 mM Glc culture condition. (B) superoxide levels were measured in WT and RIP-UCP2 islets following further acute incubation at 2.8 mM glucose (Low) and 22.8 mM glucose (Stim) after 1hr pre-incubation at 2.8 mM glucose. **p < 0.01, *p < 0.05 versus corresponding Low of the same genotype.