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. 2017 Apr 10;97(8):922–934. doi: 10.1038/labinvest.2017.30

Figure 5.

Figure 5

Hcy promoted the expression of NLRP3 protein and increased the production of IL-1β and IL-18 in macrophages. THP-1-differentiated macrophages were treated with indicates concentrations of Hcy for 24 h. Cells were subsequently harvested for western blotting, and supernatants were 32/33 collected for ELISA of IL-1β and IL-18. Data are representative of three independent experiments. (a) Representative blots and quantitative analysis of NLRP3 to β-actin. (b, c) Levels of IL-1β and IL-18 in cultured supernatant were measured by ELISA. Hcy, homocysteine. *P<0.05, **P<0.01.