Figure 2.

Gene mutations according to the degree of assay sensitivities in 240 mCRC patients. Sequenom found 57.9% of specimens mutated at RAS/BRAF (mRAS/BRAF), whereas 42.1% were wild-type (WT). A more sensitive fast COLD-PCR method recruited additional mutated sequences in both wild-type samples and in mutated samples. In particular, 90.6% of the samples RAS/BRAF WT by Sequenom were confirmed by fast COLD-PCR, whereas the remaining 9.4% of DNAs were mutated at KRAS exon 2 (ex 2) with a discrete not negligible (1–5%) or low (<1%) mutational load.